Look Micro, Think Macro: The Legacy of 40+ Years of Electron Microscopy at MLML

By Ivano Aiello (28 July 2016)

Popular science magazines and documentaries depict marine scientists as sea going adventurers equipped with a pair of binoculars or a fish net, or explorers who dive in the abyss using SCUBA or with submersibles to observe the ‘big’ things that populate the oceans.

radiolarian_S. Tanner
The invasion of the saucer man! No, this is not a picture of an alien creature but a close up (scale bar to the bottom right is 1mm) of internal structure, spines and exoskeleton of a radiolarian, a single-celled, planktonic organism that populates the waters of the Monterey Bay and builds its skeleton using silicon. Yes the same stuff that makes up the bulk of microchips. The picture was taken by Sara Tanner using MLML’s Hitachi S-3400N-II SEM.

 

Although it is true that there are some marine biologists that track the movements of humpback whales or great white sharks, and oceanographers that explore submarine canyons and other geological features which often surpass in size and majesty similar land features, not all marine biologists and oceanographers study the big things: it is quite the opposite.

coccosphere_S. Tanner
At the bottom of the food chain: this SEM photograph by Sara Tanner shows a well preserved coccosphere which is an agglomerate of tiny ‘shields’ made of calcium carbonate that single-celled marine algae called coccolithophorids.

 

The development of modern marine science in the last century has demonstrated that it is only by fully understanding the small-scale processes and features that we improve our knowledge of the vastness and complexity of the ocean’s habitats. In a sense, the small (the ‘micro’) is the new frontier to understand the big (the ‘macro’).

The numbers speak for themselves. According to recent estimates, about ½ of the global biomass (the weight of all living things) could be made of single celled microbes most of which live in or underneath the oceans. Big living things are at the top of very complex food webs while foundations are made of often very small organisms: for instance a whale can consume as much as 40 million krill per day. Big geologic features like mid-ocean ridges (the largest mountain chains on earth) are made of minerals invisible to the naked eye. And much of the sediments that fill up the ocean basins are made of the hard remains of tiny planktonic organisms that populated the oceans thousands to millions of years ago.

Pseudo-nitzscia_S. Tanner
Pennate diatoms genus Pseudo-nitzschia photographed by Sara Tanner Many species produce domoic acid, a neurotoxin which is responsible for Amnesiac Shellfish Poisoning.

 

To study the small world of the oceans, classic tools of marine science are not enough to observe and collect valid scientific data. The observation of the microscopic features of marine organisms such as corals, foraminifera, diatoms or sponges or the interior structures of organic cells (nucleus, mitochondria…etc.) requires very high magnifications, 10,000 and larger, more powerful than the optical microscopes, limited by the physics of light can yield.

copepod_S. Tanner
A primary consumer: Sara Tanner took this SEM picture of a copepod, a so called primary consumer, an intermediate link in the food chain that ends up with ternary and quaternary consumers like sharks.

 

The invention of the first electron microscope by Max Knoll and Ernst Ruska and the production of the earliest scanning-transmission electron microscope (SEM) by Manfred Von Ardenne in Berlin in the 1930s allowed scientists to finally observe the microscopic world to magnifications before unthinkable. The introduction of the first commercial scanning electron microscopes (SEMs) in 1965 opened up a new world of analysis for materials scientists.

sand particle_I. Aiello
No is not a meteorite! In paleoceanography a speck of sand can tell a lot. This one tells us a history of ancient glaciers and icebergs that occurred in the Bering Sea more than a million of years ago. Note the clear cut on the right side of the grain indicating mechanical breaking typical of glacial environments. Photo Ivano Aiello.

 

Electron microscopes are scientific instruments that use a beam of energy electrons that allow us to ‘see’ objects on a very fine scale. The electrons are accelerated by a high voltage electron gun in a cathode ray tube (yes like the one used in the old school televisions) and condensed in a beam that scans and interacts with the specimen: the interactions produces new (secondary) electrons or backscattered (primary) electrons that are captured by a detector and turned into an electrical signal. A computer analyzes the signal and based on the location of the beam and intensity of the signal converts it into an image.

Moss Landing Marine Laboratories has been at the forefront of scanning electron microscopy to study of the ultrasmall world in marine science since the very beginning of this technology. In the early 1970s, the lab acquired a Topcon SEM. It was the work of MLML’s first faculty member Dr. James Nybakken used the SEM to explore the world of marine invertebrates (James Nybakken: the first faculty member of MLML; https://anniversary.mlml.calstate.edu/2016/06/james-nybakken-the-first-faculty-member-of-mlml/). Signe Amanda Lundstrum a lab technician for Dr. Nybakken in the early 1970s served as the first SEM technician until 1989 the year when the Loma Prieta 1989 earthquake destroyed the old building.

Saving Topcon SEM after quake_L. McMasters
It was the year 1989 after the Loma Prieta earthquake that destroyed the old lab and the old Topcon SEM was carefully removed to be transferred to the trailers in Salinas.

 

After the earthquake the SEM was setup in the Salinas trailers with the help of Signe. Guillermo Moreno then replaced her for a few years and finally Sara Tanner has been the main SEM technician until 2015. While MLML was in Salinas, Sara operated an ISI SX30 SEM from 1994 until the lab moved to its present location at Moss Landing, when we purchased a Hitachi S520 in 2000.

Sara Tanner’s specialty has been to distinguish and define fine structures necessary for phytoplankton identification, and she has numerous collaborations including important studies on iron fertilization with Kenneth Coale.

New Hitachi S-3400N-II SEM at MLML
The new Hitachi S-3400N-II low-vacuum SEM at MLML. The EDX probe can be seen in the back of the electron gun.

In 2009, through an institutional grant supported by the NSF Major Research Instrumentation Program, MLML acquired a Hitachi S-3400N-II, a modern low-vacuum SEM, equipped with an X-ray spectrometer EDX. These state of the art instruments and the large sample holder (10cm) of the new SEM have significantly increased the ability of faculty, research and scientists from nearby institutions to conduct microanalysis of biological material, sediments, rocks, hard skeletal parts, and other materials.

The low-vacuum capability of the new SEM together with the X-ray spectrometer combine the power of back-scattered electron imaging with the ability to display the distribution and quantification of elements therefore the stoichiometry of the targeted material. This technique, based on the characteristic X-ray radiation produced by a substance bombarded by an accelerated beam of electrons, is fundamental to determine the composition of unknown minerals and other biological and non-biological materials at the nanno-scale.

celestite in coccolith matrix
Backscattered electron microscopy showing very bright (much denser) crystals of celestite (strontium sulfate) embedded in a matrix of mostly dissolved coccoliths of Pliocene and Pleistocene age. This specimen comes from the fluid mud floating in the Urania Basin, one of the first deep water hypersaline anoxic basins ever discovered. Photo Ivano Aiello.

 

Since 2009, there have been many examples of multidisciplinary studies done by faculty and students in many labs within and outside MLML.

Undoubtedly this relatively new instrument with its incredible imagining and compositional capabilities will give further opportunities to investigate the micro-world of marine sciences and will inspire future generation of MLML’s students.

Special thanks to Sara Tanner and Lynn McMasters for helping with pictures and stories for this blog!

Deepsea mud_I. Aiello
Deep marine sediments in the Bering Sea. Some people call it mud, but there is much more to it. In places of high primary productivity like the Bering Sea, the ‘mud’ is made by hard parts of dead marine plankton (mainly diatoms) that settle out from the ocean surface, blanket the seafloor and fill up entire ocean basins. Photo Ivano Aiello.

 

 

 

The K-T Boundary of Moss Landing Marine Labs

By Gary Greene ( 21 July 2016)

Bathymetry in Monterey Bay
Bathymetric image from Google (in blue colors) and MBARI (orange to purple colors) of the Monterey Canyon system that heads at Moss Landing, one of the worlds most studied submarine canyons; blue dot is location of MLML.

 

The Cretaceous-Tertiary (K-T) boundary in geologic stratigraphy marks a seminal time in geologic history, a time when dinosaurs and other organisms were extinguished from the surface of the earth and the rise of new genera and species occurred. A similar type of evolution can be said to have occurred at the Moss Landing Marine Labs, a time line marked by the earthquake of 1989.

Earthquakes are not unusual along active plate boundaries such as the one that MLML sits on, but they are always a surprise when they occur. This of course is what makes MLML such an attractive place to study marine geology and has attracted faculty and students to the place in the past 50 years. Situated on the Pacific-North American plate boundary at the continents’ edge and overlying a block of Cretaceous granite known as the Salinian Block, the Labs’ geographic location has been transported from where Santa Barbara is located today to its present position since the mid-Tertiary time, in the past 27 million years.

faults along western CA
The San Andreas fault (SA) lays to the east of Monterey Bay with the western side moving northward and the eastward side moving southward. MLML is located on the Salinian Block of granite (in yellow).

 

The dynamic edge of the continent is expressed in many faults mapped both onshore and offshore. In the Monterey Bay region the San Andreas is the master plate boundary fault, rupture along which produced the 1989 earthquake, but just offshore of MLML in Monterey Bay are several other active faults, faults of the San Gregorio and Monterey Bay fault zones that control the geomorphology of Monterey Canyon and part of the San Andreas fault system.

Fault systems in Monterey Bay
Multi-beam echo sounder bathymetric image of the seafloor offshore of MLML in Monterey Bay showing locations of faults and fault zones associated with the San Andreas Fault system, a major tectonic plate boundary, and the fault offset geometry of Monterey Canyon.

 

Monterey submarine canyon, eroded deeply into the granitic rocks of the Salinian Block, sits just offshore of Moss Landing and is the largest such feature along the contiguous U.S. It is the size of the Grand Canyon of the Colorado River and its active heads are a stone throw from the beach. Here the canyon heads intercept sand transported along the coastal nearshore areas and sends the sediment down to the abyss. Cold nutrient waters upwell in its heads inviting in marine fauna including whales that feed and frolic in its waters. Fran Shepard, the father of Marine Geology, recognized the geological significance of this canyon in the 1930s and it has been intensively studied ever since, being the most studied canyon in the world. Scientific contributions from students, faculty, and researches at MLML often in cooperation with colleagues at the Monterey Bay Aquarium Research Institute have furthered the understanding of submarine canyon processes.

Monterey Submarine Canyon head at MLML
The Monterey Submarine Canyon has a number of heads that abut Moss Landing. The intake for the MLML seawater system sits offshore of MBARI (to the northwest of the MLML pump house), on a small plateau between two heads of the canyon. The red star represent the location of the old lab prior to the 1989 earthquake.

 

From 1966, when I first came to the Labs to study marine geology, to 1989, MLML resided in a converted cannery and the former Beaudette Foundation’s marine laboratory on the Moss Landing Spit, with the ocean waves lapping at its backside. The library, hosting wood paneling, a fire place, and windows to the sea was a warm, ideal, and inviting snug place in which to learn, and was lovingly overseen by one of the most gracious and welcoming librarians I have ever known, Sheila Baldridge. After the Labs were expanded in the 1980s, the library was moved to an upstairs location in the old cannery building, again overseen by Sheila, where Director John Martin provided the first USGS Pacific Marine Geology field office, which I was able to occupy until the earthquake hit and pulled the Labs apart.

The earthquake, of course, destroyed this quiet, bucolic setting, but it had fostered a hidden strength in its students, faculty and staff, and the community that rose to tackle the rebuilding of the Labs. From the dust, or should I say from the sand boils and liquefaction, was born the: “Friends of Moss Landing Marine Labs,” “Back-to-the Bay” movement, “Save the Water Tower,” and the fight to build a new laboratory, despite “Sally,” Noel Mapstead, and Native American objections to rebuilding the Labs on “The Hill.”

post_quake (4)
MLML after the 1989 Loma Prieta earthquake.

 

After the earthquake, faculty, staff and students moved to a temporary trailer campus in Salinas, far from the sea and where chocolate permeated the air rather than salt spray. It is a wonder that anyone teaching, studying or working at the Salinas facilities can eat chocolate today. Nevertheless, all persevered with each and everyone connected to, or in support of, the Labs (far too many people to list here), in good faith and selflessly, sometimes on conflicting courses, undertook personal and team efforts to return the Labs to Monterey Bay and to build a lasting institution for the coming generations of students.

Salinas Trailers 7
The trailers that comprised MLML for ten years on Blanco Rd, Salinas.

 

It was my privilege to come to the Salinas Labs in 1994 as Director to assist in guiding through the hurdles in the path to the sea. Cloaked in the likes of a larvacean house, a fitting costume for any of the Labs’ Halloween parties at Elkhorn Yacht Club, I appeared on the scene with the intent to filter out the bull s___ (B.S.) that falls from above and pave a smooth path forward. I was pleased to receive considerable support and with this support we were able to turn back opposition. In spite of finding Native American midden remains, and a multitude of legless lizards on the hill, the Labs received the funding necessary for reconstruction (thanks at least partially to the David and Lucile Packard Foundation). The new MLML facility was completed after nearly a decade of struggle.

The time line (MLML’s K-T boundary) was transcended once the new facilities were in place. The faculty had designed a magnificent building, well laid out for teaching and research. It now stands as the window into the ocean and from its expansive backside overlooks the Pacific and the head parts to Monterey Canyon, where geological processes and active marine mammal and bird activity can be observed first hand. Survival of the fittest occurred and new generations of faculty, students and staff are carrying the Labs across its K-T boundary and into a future with great promise. Being on the MLML scene 50 years ago seems like yesterday, but I am a geologist and 50 years is the blink of the eyelid in geologic time.

cloud point of MLML main building
Cloud Point image of the main building of MLML on the hill based on a recent UAV flight.

 

 

 

 

The Pacific Shark Research Center (PSRC)

By Joseph J. Bizzarro (14 July 2016)

Sharks are sexy, sure – but skates and chimaeras are sexy too. You want proof? Well, good (or too bad, because here it comes). This week’s blog is about MLML’s Pacific Shark Research Center, an extremely productive program that was initiated in 2002 and has graduated 25 students and produced > 250 conference presentations, 180 peer-reviewed publications, and 22 books.

Founders of PSRC
PSRC Staff Members (from L to R) Wade Smith, Joe Bizzarro, Greg Cailliet, and Dave Ebert. It’s unclear why Joe and Dave got to hold the big jaws while Wade and Greg were stuck with the wee bits.

 

Back at the turn of the century, three prominent shark research programs at Mote Marine Laboratory, the Virginia Institute of Marine Science, and the University of Florida were working to secure federal funds to conduct biological research on elasmobranchs (sharks, skates, and rays) with direct applications for fishery management. East coast populations of several shark species were in trouble, mirroring a global trend of severe declines in many species – especially those that are extremely k-selected and/or subject to intense exploitation – and shifts in the composition of skate assemblages. The greatest federal advocates of the program, however, were congressional representatives from California. This was fortuitous for Greg Cailliet, who had a long professional and personal history with the major players from each of the east coast institutions and had carved out his own prominent elasmobranch research program at MLML. Phone calls were made, many long emails were surely exchanged (this is Greg we’re talking about), and behold. In 2001, Congress approved $1.5 million in funding to the National Shark Research Consortium through the National Marine Fisheries Service’s (NMFS) Highly Migratory Species program. With this award the only west coast representative, the Pacific Shark Research Center (PSRC), was born.

Bert_M. Boyle_J Kemper_K James
Dave Ebert instructs PSRC students (L to R) Mariah Boyle, Jenny Kemper, and Kelsey James in the fine art of catshark identification.

 

At establishment, the PSRC consisted of three staff members in addition to Greg, who served as the Program Director: David Ebert, who acted as Program Manager, and Project Managers Joe Bizzarro and Wade Smith. For the first few years, Joe and Wade were still toiling on their MS degrees while also working for the PSRC. The influx of funding also helped to support MS research for Greg’s ichthyology students who were interested in elasmobranchs, and the targeting of new students whose interests aligned with the goals of the program. These goals were to conduct basic and applied biological research on chondrichthyan fishes (elasmobranchs plus chimaeras, a poorly known sister group to the sharks, skates, and rays), establish the PSRC as a resource center for scientific information on chondrichthyans to public policy makers, provide scientific expertise to NMFS and state management agencies to help better monitor and manage chondrichthyan fisheries off the U.S. Pacific Coast and in Alaskan waters, and participate in collaborative research on national and international issues involving shark, ray, and chimaera biology. Research was focused on addressing major gaps in our understanding of the life history (age, growth, reproduction, and demography), stock structure, ecology, and fishery biology of commercially and recreationally important chondrichthyan species.

Lewis Barnett and a Big Skate
Former PSRC student, Lewis Barnett, hoists an aptly named big skate (Beringraja binoculata) during fieldwork in the Gulf of Alaska.

 

“Shark” is a great buzz word – appealing to funding agencies – but in practice, the PSRC could have been dubbed the Pacific Skate Research Center, as the majority of the research has focused on this group of batoids (skates and rays). Skates are exploited in commercial groundfish fisheries throughout the world’s temperate and boreal regions, primarily as bycatch in other fisheries. Despite this incidental take, fishery mortality has altered species composition of skates and caused substantial declines in the populations of many large, nearshore species. Skates were afforded little scientific or management attention in the past because they have not supported lucrative or sustained fisheries. However, this situation is changing because skates are predators and competitors of other commercially important groundfishes, and because dramatic changes in the population sizes of exploited species have occurred. In the water off the U.S. Pacific Coast and in Alaska, management regulations either have curtailed or severely reduced commercial shark landings, but skate bycatch remains a major problem throughout the region, and skate management is therefore a primary concern.

PSRC open house_1
Recent PSRC students at Open House: L to R: Matt Jew, Amber Reichert, Justin Cordova, Vicky Vasquez, and Paul Clerkin.

 

Skates are represented by nearly 300 species of benthic, egg-laying cartilaginous fishes that constitute one-quarter of all extant chondrichthyans. Although they are extremely speciose, skates have conservative morphology, consisting of a dorso-ventrally flattened body and a limited color pallet that includes shades of brown, grey, and black. Skate identification therefore is difficult, and skate species have been historically misidentified or grouped into generalized categories by fishery scientists and managers for convenience. Describing new skate species and addressing current identification problems  therefore has been a research priority of the PSRC.

Broad Skate
Skates have historically been associated with soft seafloor regions consisting of silt, mud, and/or sand. However, recent spatial studies by PSRC personnel and others, and in situ video like this image of the broad skate, Amblyraja badia, at the Davidson Seamount (1641 m), have revealed much more diverse and complex habitat associations.

 

Although species-specific identification has been problematic, some general characteristics of skate biology have emerged. Skates are extremely widespread, ranging throughout the globe from intertidal regions to the abyssal plain. They typically have k-selected life histories, a trait that is especially pronounced in species that attain relatively large sizes and those that inhabit deep-water. The ecological interactions of skates are not well understood, but they historically have been considered to be generalist predators that occur mainly on soft-bottom regions. PSRC research has helped to advance our knowledge of these aspects of skate biology, and more publications on skates have been produced since 2002 by PSRC personnel than by any other group in the world.

whiteblotched skate
Generalized or erroneous identification and a lack of scientific attention have resulted in a poor understanding of distribution and abundance patterns of eastern North Pacific skates. This and several other whiteblotched skates (Bathyraja maculata) were recorded by PSRC personnel off SE Alaska in 2006, representing an eastern range extension of > 1000 km.

 

The salad days of the PSRC stretched from 2002–2009. During that time, federal funding was consistent and ranged from 1.5 to 2.0/year, split roughly equally among the four NSRC institutions. Federal funding was, however, terminated in 2009, and coincided with substantial change in the composition and focus of the PSRC. In 2008, Wade left to pursue a PhD at Oregon State University. Joe hung around until the funding ran out in the summer of 2009, then matriculated at the University of Washington during the 2009 to conduct his own PhD. In addition, Greg retired at the end of the 2009 academic year.

Since 2009, the PSRC has mainly functioned under the direction of Dave Ebert on shoestring budgets, as no steady or substantial source of direct funding has been available. The main objectives of the program remain the same, but Dave began to focus more effort on discovering “Lost Sharks,” poorly known or unidentified/misidentified species and especially those that are exploited in commercial fisheries. This focus builds on some of the major misconceptions of chondrichthyan fishes. The public’s perception of sharks often conjures up images of a large, fearsome, toothy predator, with its large dorsal fin cutting its way through the waters’ surface. However, the reality is that sharks come in a variety of sizes and shapes, from the whale shark (Rhincodon typus), the world’s largest fish, to the dwarf pygmy sharks (Squaliolus spp.). In addition, the batoids and chimaeras have historically received considerably less scientific attention than sharks, but are similarly exploited directly as fishery targets, or indirectly as bycatch.

Unknown shark
A Sculpted Lanternshark (Etmopterus sculptus), a species described by David Ebert in 2011. Paul Clerkin photographed these little sharks during a survey in the Southeastern Atlantic. This project was done in collaboration with the United Nations’ Food and Agriculture Organization (FAO) and the Southeast Atlantic Fisheries Organization (SEAFO). Paul was very excited to be included as the ship’s shark expert and help identify shark species to assess the area’s vulnerability to fishing pressure.
E scultus by Jacque Orvis
Head of a Sculpted Lanternshark (Etmopterus sculptus). Photo by Paul Clerkin.

 

Our awareness of the diversity of sharks and their relatives has increased substantially in contemporary times, with more than 240 new species described over the past 15 years. This represents nearly 20% of all shark species that have been described throughout human history. Most of these new discoveries have come from the Indo-Australian region, followed by the Western Indian Ocean and western North Pacific regions. However, despite such a rich and diverse fauna, the majority of sharks and their relatives have largely been “lost” in a hyper-driven media age whereby a few large charismatic shark mega-stars overshadow the majority of shark species. While these mega-star’s, such the great white shark (Carcharodon carcharias), receive much media adulation and are the focus of numerous conservation and “scientific” efforts the “Lost Sharks” remains largely unknown not only to the public, but also to the scientific and conservation communities.

little sharks
Andrea Launer with young leapard sharks.

 

Currently, Dave advises 11 graduate students that comprise the PSRC. Joe is back from UW, working with Mary Yoklavich at the NMFS facility in Santa Cruz, and Greg is enjoying his retirement and no longer directly involved in the day-to-day operations of the PSRC. Greg and Joe do, however, collaborate regularly with Dave and his students and have several research projects in progress on chrondrichthyans. The hope is that Dave’s Lost Sharks program will garner the attention of an interested funding agency, restoring a much needed financial infusion to the program. Regardless though, in true MLML fashion, Dave continues to work (gratis) for the PSRC in order to educate MLML students and continue to build our knowledge base about chondrichthyans. The accomplishments and productivity of the PSRC are considerable (see below) and speak to the dedication of the staff and students involved, and their love of these ancient cartilaginous fishes.

trawler_Paul Clerkin
Paul Clerkin took this shot of the deck on the fishing vessel Will Watch as the crew was preparing to deploy their deep-sea trawl net (note the people in the orange slickers). Paul spent two weeks aboard this ship studying sharks caught as bycatch. During this survey Paul discovered a number of new shark species which another Pacific Shark Research Center student and he are currently describing with the help of David Ebert.

 

Paul Clerkin
Paul Clerkin holding what is believed to be a new species of sleeper shark. This is the only specimen of the species, and we know absolutely nothing about these interesting animals. Photo by Jacqueline-Remy Orvis.

 

PSRC Facts:

  • 25 MLML/PSRC graduate students completed their degrees; 13 of these students have gone on and enrolled into Ph.D. programs with 5 having completed their Ph.D.
  • Since inception the PSRC has conducted >100 research projects, mostly in the California Current, Gulf of Alaska, and Eastern Bering Sea large marine ecosystems, but also in collaboration with colleagues in Canada and Mexico.
  • During this project period we have produced approximately 700 publications (average 56/year) including those that have been published, are in press, or are currently in review; this includes 22 books.
  • PSRC Students were lead or co-authors on approximately 350 of these publications.
  • PSRC students averaged 10 publications each of all kinds including book chapters, IUCN Red List Assessments, popular articles and electronic on-line publications.
  • PSRC staff and students published 180 papers (average 14.4/year) in peer-reviewed professional journals.
  • Individual PSRC graduate students averaged 3 peer-reviewed publications each.
  • The PSRC has contributed to about 130 IUCN Red List Assessments.
  • The National Shark Research Consortium received $11,088,174 in Federal funding from 2002-09.
  • $1,400,000 in extramural funding was additionally secured by PSRC personnel, which helped support graduate students at MLML and provided additional support for field work and travel.
  • 25 MLML Graduate students were partially or fully supported.
  • PSRC staff and students attended over 80 professional conferences and gave 250 presentations.
  • PSRC personnel delivered the keynote address at 10 International Conferences.
  • Six PSRC students won individual conference presentation awards for best student presentation.
  • The PSRC has named 30 new species of chondrichthyans, making us the 2nd leading institution globally for discovering and naming new species.
  • The PSRC has discovered 5 chondrichthyan species from off the California coast that had not previously been known.

 

 

 

Friends lecture Wednesday July 20th at 7pm

Come spend the evening at Moss Landing Marine Labs to hear Jason Adelaars speak about a few of the projects happening at Moss Landing Marine Labs. Learn how we are using cutting-edge technology to remove nutrients from agricultural run-off, identify areas vulnerable to the impacts of sea level rise around Monterey Bay, and create real-time oceanographic monitoring to achieve results. The lecture starts at 7:00pm and will be in the main seminar room. Recommended donations for non-friends of MLML is $8. If you are unsure if you are a friend of MLML you can check by calling 831-771-4100 or emailing friends at friends@mlml.calstate.edu. Hope to see you there!
 

MLML Student Life in the 1990s

By Erica J. Burton ( 8 July 2016)

Being a MLML student during the post-earthquake 90s meant learning, working, studying, and maybe living (caretakers) in the Salinas trailers (Fig. 1). Sitting in the middle of agriculture fields may have made a student question their recent arrival and admission to the lab. However, the Salinas office staff (including Gail Johnston and Sandy Yarbrough) made students feel welcome and connected to their home campuses. The Librarians, Sheila Baldridge and Sandy O’Neill, took great interest in the students and their projects; and could always retrieve the most obscure, needed references. And the faculty had an energetic quality that inspired, united, and promoted the student body.

Fig_01_MLML_trailer_study_room
Figure 1. Tomo Eguchi and Erica Burton doing Statistics Class homework in Salinas trailer study room (which also served as journal and map storage, TV room, computer lab, copier room, snack and soda machine vending, and lounge area).

 

As a first-year student, you may have attended a core class (or two) in the triple-wide trailer on the island in Moss Landing (neighbors to the bait & tackle shop, and the original Phil’s Fish Market (Fig. 2); land now occupied by the MLML aquaculture facility).   Also on the island was (is) the MLML Small Boat and Diving Operations building (Fig. 3); a student’s gateway to small boat usage in Elkhorn Slough or SCUBA diving field work (Fig. 4).

Fig_02_Phils_Fish_Market
Figure 2. Phil’s Fish Market where lunches or fish specimens were acquired (1992).

 

Fig_03_MLML_small_boats
Figure 3. MLML Small Boats and Diving operations (1992). Gateway to Elkhorn Slough and SCUBA diving fieldwork.

 

Fig_04_diving_90s_2
Figure 4. John Heine’s Dive Class aboard R/V Ricketts. Pictured left to right: Tony Orr, Erica Burton, Michele Jacobi, Matt Edwards.

 

Although the temporary MLML campuses were separated by ~16 miles, the MLML community was tight-knit, and chock-full of camaraderie and can-do attitudes. There was nothing we couldn’t do (or at least try). Close-quarters in the trailer labs may have aided our tight-knit community, as shown in the Ichthyology Lab (Fig. 5).

Fig_05_MLML_ich_lab_ageing_mod
Figure 5. Fish-ageing quarter of the Ichthyology Lab Trailer. Pictured left to right: Korie Johnson, Schaeffer, Dawn Outram, and Julie Neer.

 

Class projects and thesis work brought students together. Students were always willing to lend a hand; near or far. It could have been an ecology class project collecting rocky intertidal fishes, manipulative experiments in Stillwater Cove, class cruises aboard the R/V Point Sur and R/V Ricketts (Figs. 6, 7), or convincing excuses to conduct field work in Baja California, Mexico (Fig. 8).

Fig_06_MLML_ecology_cruise_1993
Figure 6. Fall 1993 Ecology Class cruise aboard R/V Point Sur (posing on dock). Pictured: Back Row, left to right: Jon Kao, unknown, Dr. Andrew DeVogelaere, unknown, unknown, Jonna Engel, Elaine Herr, Dave Lindquist, Michelle White, Sean McDermott, Lisa Kerr Lobel, Erica Burton, unknown; Front Row, left to right: Kit Muhs, Dr. Gregor Cailliet, Noel Cristimoto (?), Barbie Byrd, Korie Johnson Schaeffer, Rebecca Reuter, Eli Landrau Woodvine, Leigh Nerney, and Bill Leopold.

 

Fig_07_MLML_Ricketts_cruise
Figure 7. Fall 1993 Fisheries Class cruise aboard R/V Ricketts. Pictured left to right: Heather, Leigh Nerney, Erica Burton, Dr. Gregor Cailliet, Dawn Outram, and Karl Mayer.

 

Fig_08_Baja_group
Figure 8. Spotted Sand Bass ageing fieldwork crew in Bahia de Los Angeles, Baja California, Mexico during Spring Break 1994. Pictured left to right: Shirley Andrews, Cheryl Baduini Zaricki, Korie Johnson Schaeffer, Tony Bennett, Tanya Sozanski Bennett, Erica Burton, Lara Ferry, Lisa Kerr Lobel, Doreen Moser Gurrola, and Allen Andrews.

 

Graduate school kept us busy days, nights, weekdays, and weekends. But those who worked hard also found time to let off steam at house parties, local watering holes, extracurricular sports, poker games, bus rides to a SF baseball/football game, ski trips, etc. The Blue House (Fig. 9) was a good place to start or end an evening; and typically involved a crooked stroll to, and from, Ray’s (aka The Moss Landing Inn). House parties may have had a live band, and there was almost always dancing involved. Monterey hot spots included Doc Ricketts, Players, Planet Gemini, Blue Fin Billiards, karaoke at the Marriott, Mucky Duck, and $2-Tuesdays at the Dream Theater (Fig. 10). The annual Bowling Tournament among faculty, staff, and students was always a big hit (Figs. 11, 12). And occasionally, faculty would host a their own lab party (Fig. 13).

Fig_09_MLML_blue_house_party
Figure 9. Dancing at Blue House party. Pictured left to right: Jonna Engel, Karen Crow, unknown, Mark Pranger, and Lara Ferry.

 

Fig_10_Dream Theater
Figure 10. Dream Theater marquee, Monterey.

 

Fig_11_MLML_bowling_Heine_Johnson
Figure 11. Dive Instructor John Heine and Dr. Ken Johnson at Bowling Tournament, looking confident in claiming bowling trophy. In background: Michelle Lander, Michele Jacobi, Cheryl Baduini Zaricki, Brendan Daly, and MLML students.

 

Fig_12_MLML_bowling_BUI
Figure 12. Team B.U.I. at 1997 Bowling Tournament. Pictured left to right: Pat Iampietro, Matt Edwards (with arm in sling; what a trouper!), Erica Burton, and Stewart Lamerdin.

 

Fig_13_MLML_Cailliet_court
Figure 13. Dr. Greg Cailliet holds court at his Ichthyology Lab house party. Pictured left to right: Rick Starr, Greg Cailliet, Lisa Ziobro de Marignac, Allen Andrews, Joe Bizzarro, and Jean de Marignac.

 

During the 90s, there was a constant effort to rebuild the lab; especially for the faculty and staff. But the students were involved, too. It was part of our psyche. There were awareness campaigns; Open House events to let the public know we were still part of the community and that we’d return to Moss Landing; visits to the Salinas courthouse for hearings; and finally celebratory events on the hill (Fig. 14).  The new lab opened in January 2000. Many of the students during the trailer years would never occupy the new lab. But, I think many would agree, the 90s weren’t about the lack of a permanent lab structure; they were about the MLML spirit, quality of education, and long-lasting friendships that were made during our Salinas years.

Fig_14_MLML_groundbreaking_1997_mod
Figure 14. MLML Ground Breaking ceremony on the hill, September 1997. Pictured left to right: Allen Andrews, Korie Johnson Schaeffer, Brendan Daly, Ned Laman, and Tony Orr (holding souvenir shovel, “MLML, We’re Back!”).