A grant from the Gordon and Betty Moore Foundation to develop a protocol for the electroporation-based transformation of toxic Pseudo-nitzschia species, which will enable future molecular physiology studies revolved around domoic acid production.
Pseudo-nitzschia transcriptome/ DA metabolism
Ongoing efforts towards genome annotation? Whatever you want to say about that
Don't know if we want to summarize this project for prosperity?
A continuation of our Alliance for Coastal Technologies partnership which involves the assessment of several commercially available fluorometers. MLML will serve as one of the test sites for the instruments, with the goal of providing performance evaluations for researchers and stakeholders.
ARISA/IFCB at Santa Cruz wharf
In 2017, we will begin a new project (in conjunction with researchers at UCSC and Florida Fish and Wildlife) that couples real-time cell/particle detection via an imaging flow cytobot (IFCB) with a genetic fingerprinting technique (ARISA) that deciphers Pseudo-nitzschia species beyond current methods used for routine monitoring. This approach will afford us an unprecedented opportunity to assess the transition of the Pseudo-nitzschia community as a bloom is initiated, and to correlate shifts with supporting environmental parameters on a high-resolution temporal scale in order to better understand what drives species to increase in cell abundance and/or toxicity.
Weekly phytoplankton monitoring at the Monterey Municipal Wharf II, supported by CenCOOS, as part of a coast-wide network of California sampling sites. These data are used in conjunction with ancillary measurements (water quality parameters, climactic observations) and results from weekly monitoring at the Santa Cruz wharf (Kudela lab, UCSC) to develop a holistic picture of long-term trends in Harmful Algal Bloom species (including Pseudo-nitzschia).